Application Notes

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Application Note

Plant Chemiluminescence

Chemiluminescence imaging requires the highest sensitivity and long exposure time in order to be able to detect the few photons emitted by the chemical reaction of interest with the living organisms.

Dr. Colleen Doherty uses a Raptor Photonics Eagle V camera in her study of the circadian rhythm in plants. Using Arabidopsis modified to express firefly luciferase Photinus Pyralis on a known heat shock gene she was able to observe unexpected expression in the roots of the plants. Both images were taken with 5min exposure and are about 45min apart before and after the heat shock. The increased luminescence is clearly visible in the roots.

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